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Guineais a poor country of approximately 12.9 million people in 2016 that possesses the world's largest reserves ofbauxiteand largest untapped high-grade iron ore reserves, as well as gold and diamonds. In addition,Guineahas fertile soil, ample rainfall, and is the source of several West African rivers, including the Senegal, Niger, and ...

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Animals. Male and female Hartleyguinea-pigs weighing 140–280g (n = 69) were attained from South Australian Health and Medical Research Institute and randomly assigned to experimental groups.Allguinea-pigs were housed in a temperature-controlled environment with 12 hour day/night cycles and free access to food and water.

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The giPScelllines were cultured in media containing leukemia inhibitory factor andguineapig fibroblastcellswere used as feedercells. These cultures were expanded under feeder‑free culture conditions using ESGRO Complete Plus Clonal Grademediumcontaining 15% fetal bovine serum on gelatin‑coated dishes.

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